Professional Serum 5ml

Whether you’re using Professional Serum for the first time or looking to get the most from every ampoule, here’s everything you need to know—from why it’s pink to the best ways to use it.

1. What is CALECIM^® Professional Serum and who is it for?

CALECIM^® Professional Serum is our most advanced regenerative skincare serum, formulated with the highest concentration of PTT-6^® in the CALECIM^® skincare range. PTT-6^® is a proprietary blend of growth factors, proteins, cytokines, and exosomes derived from ethically sourced umbilical cord lining stem cells. While the serum contains no stem cells, it delivers thousands of naturally occurring bioactive cell-signaling proteins in every pipette to help support your skin’s natural renewal processes.

Originally developed for use in aesthetic clinics, Professional Serum can be used in three ways:

• As part of your daily skincare routine to support your skin’s ongoing renewal and repair processes against everyday environmental and internal stressors associated with skin aging.


• To complement at-home microneedling, helping optimize your skincare routine before and after treatment.


• To support recovery following professional aesthetic procedures, when your skin’s natural repair response has already been activated by treatments such as microneedling, laser resurfacing, or chemical peels.

Professional Serum is ideal for anyone looking to improve visible signs of skin aging, including fine lines, wrinkles, loss of firmness, uneven skin texture, and dull-looking skin. It is also an excellent choice for those who want professional-strength skincare to complement in-office treatments or maintain healthier-looking skin between procedures.

2. How do I use Professional Serum in my skincare routine? Should I apply it before or after moisturizer?

Professional Serum should always be applied after cleansing and before moisturizer.

There are three ways to incorporate it into your skincare routine:

Daily skincare

Apply one pipette to clean skin once or twice daily before your moisturizer. This helps support your skin’s natural renewal process every day while defending against the visible effects of aging.

With at-home microneedling

Apply two pipettes before microneedling to provide glide during the treatment, followed by two pipettes immediately afterward. You may then apply CALECIM^® Recovery Night Complex to help cool, hydrate, and support the skin barrier during recovery.

With professional aesthetic treatments

Many patients bring an ampoule with them when undergoing treatments such as microneedling or laser resurfacing. Up to half an ampoule may be applied during and immediately after the procedure. The remaining serum can then be used at home by applying one pipette daily over the following days to help optimize your skin’s recovery.

Allow the serum to absorb completely before applying moisturizer. During the day, finish your routine with a broad-spectrum sunscreen.

3. Should I use Professional Serum in the morning, at night, or both?

Professional Serum can be used morning, evening, or both, depending on your skincare goals.

Many people choose to use one pipette once daily, allowing each ampoule to last approximately two weeks. Others prefer to use it morning and night for more intensive daily care.

Whichever routine you choose, consistency is the key to achieving the best results.

4. How much Professional Serum should I apply?

The amount depends on how you’re using the serum.

• Daily skincare: One pipette per application.


• At-home microneedling: Two pipettes before treatment and two pipettes immediately afterward.


• Professional aesthetic treatments: Up to half an ampoule may be used during and immediately after your procedure, with the remaining serum applied at home over the following days.

Using the serum consistently is more important than applying larger amounts.

5. Can I use Professional Serum around the eyes, neck, and décolleté?

Yes. Professional Serum can be applied to the face, eye area, neck, and décolleté—areas that are often among the first to show visible signs of aging.

Avoid direct contact with your eyes.

6. How long does one ampoule last?

Each ampoule contains approximately 12–14 pipettes of Professional Serum, so how long it lasts depends on how you use it.

• Daily skincare (one pipette per application): Approximately 12–14 applications, or about 1–2 weeks, depending on whether you use it once or twice daily.


• At-home microneedling: Approximately three complete microneedling sessions, using two pipettes before and two pipettes after each treatment.


• Professional aesthetic treatments: If approximately half an ampoule is used during your procedure, the remaining half typically provides 6–7 daily applications to continue supporting your skin’s recovery at home.

7. How should I store Professional Serum? Do I need to refrigerate it?

Store Professional Serum in a cool, dry place away from direct sunlight and excessive heat.

Refrigeration is not required. Simply replace the cap securely after each use to help preserve the quality and stability of the formulation.

8. Why does Professional Serum have its distinctive color and scent?

Professional Serum contains a very high concentration of biologically derived PTT-6^® and is intentionally fragrance-free.

Because it is frequently used immediately after aesthetic procedures and on skin with a temporarily compromised skin barrier, we chose not to add fragrance. This minimizes unnecessary ingredients that may increase the risk of irritation in sensitive or post-procedure skin.

Its characteristic scent comes naturally from the bioactive ingredients rather than from added fragrance.

The serum’s pink color is not an artificial dye. It serves as a built-in pH indicator, providing an additional quality control measure to help ensure the biological proteins remain stable and active.

Because PTT-6^® is rich in naturally derived proteins, prolonged exposure to excessive heat may occasionally affect the formulation. In rare cases, this can alter the pH, causing the serum to become colorless, yellow, or milky.

If you notice a significant color change, discontinue use and contact us through the website chat widget with a photo of your product and your order number. Our team will be happy to assist you.

9. How can I get the best results from Professional Serum?

For the best results, begin with thoroughly cleansed skin. Remove makeup, sunscreen, excess oil, and surface impurities before application so the bioactive ingredients can come into direct contact with your skin.

Apply Professional Serum immediately after cleansing and before any other serums, moisturizers, or facial oils, allowing it to absorb fully before continuing with the rest of your skincare routine.

To maximize your results:

• Cleanse your skin thoroughly before every application.


• Apply Professional Serum before all other leave-on skincare products.


• Use it consistently as part of your daily skincare routine.


• Apply sunscreen every morning to help protect your skin from ongoing environmental damage.


• If you’re using Professional Serum alongside microneedling or professional aesthetic treatments, continue applying the remaining serum during the days following your procedure to help support your skin’s natural recovery process.

Like exercise and healthy nutrition, consistent long-term use delivers the greatest visible improvements over time.

Professional Serum fits effortlessly into both your everyday skincare routine and your post-procedure recovery plan. Discover how to pair it with your favorite skincare products and professional treatments.

1. Why do aesthetic clinics use Professional Serum after skin treatments?

Professional Serum was originally developed for use by physicians and aesthetic practitioners as part of post-procedure skincare.

Treatments such as microneedling, laser resurfacing, radiofrequency microneedling, and chemical peels work by creating controlled micro-injuries that stimulate the skin’s natural repair response. During this recovery phase, the skin is highly active as it begins rebuilding and renewing itself.

Professional Serum is frequently incorporated into these treatment protocols because it delivers a high concentration of PTT-6^® bioactive signaling molecules—including growth factors, proteins, cytokines, and exosomes—to help support the skin’s natural renewal process during this critical recovery period.

Many practitioners continue recommending Professional Serum for home use following treatment to help extend post-procedure skincare as the skin continues to recover over the following days.

2. Can I use Professional Serum before or after aesthetic treatments such as microneedling, laser resurfacing, chemical peels, RF microneedling, dermabrasion, Botox, or dermal fillers?

Yes.

Professional Serum is commonly used alongside many aesthetic procedures and can be incorporated into both professional treatment protocols and your at-home recovery routine.

For treatments that create controlled micro-injuries—such as microneedling, laser resurfacing, chemical peels, RF microneedling, and dermabrasion—the serum is often applied during the procedure and immediately afterward. Continuing to use the remaining serum at home over the following days helps support your skin’s natural recovery process.

For injectable treatments such as Botox and dermal fillers, Professional Serum can also be incorporated into your regular skincare routine. Your healthcare provider will advise you on the most appropriate timing following your procedure.

Always follow your provider’s post-procedure instructions, as recommendations may vary depending on the treatment performed.

3. How soon after a skin procedure can I begin using Professional Serum?

The appropriate timing depends on the procedure you’ve had and your provider’s treatment protocol.

For many in-office procedures, Professional Serum may be applied immediately following treatment by your physician or aesthetic practitioner. If you are taking your ampoule home, continue using it exactly as instructed by your provider.

If your skin has been treated elsewhere, always follow the post-procedure instructions provided by your healthcare professional before introducing any skincare products.

4. Can I use Professional Serum with retinol, vitamin C, exfoliating acids, peptides, prescription skincare, and other active ingredients?

Yes. Professional Serum is designed to complement a wide variety of skincare routines and can generally be used alongside ingredients such as retinol, vitamin C, niacinamide, peptides, hyaluronic acid, exfoliating acids, and prescription skincare products.

Professional Serum was originally developed for use in aesthetic clinics and is frequently incorporated into post-procedure treatment protocols by physicians and aesthetic practitioners. If you have recently undergone a professional skin treatment or are using prescription skincare, it is always best to follow the recommendations of your treating physician or skincare professional regarding which products should be used during your recovery.

When layering skincare products, apply Professional Serum immediately after cleansing and before your other serums, moisturizers, or facial oils.

5. Can Professional Serum be used on sensitive skin?

Yes.

Professional Serum is suitable for sensitive skin and was originally developed for use on skin recovering from professional aesthetic procedures, when the skin barrier may be temporarily compromised.

Its fragrance-free formula and high concentration of naturally derived PTT-6^® bioactive proteins have made it a trusted choice among physicians and aesthetic practitioners as part of post-procedure skincare protocols. It is designed to support the skin’s natural recovery process while helping maintain a gentle skincare routine during this important stage.

If you have a known allergy or a highly reactive skin condition, we recommend consulting your healthcare provider before use.

6. Can Professional Serum be used on acne-prone skin?

Professional Serum is not recommended for skin with active inflammatory acne or open acne pustules, as compromised or inflamed acne lesions may be more reactive to topical skincare products.

We generally recommend waiting until active acne has settled before introducing Professional Serum into your skincare routine.

However, Professional Serum may be incorporated into certain physician-directed treatment protocols designed to improve the appearance of post-acne scarring and support skin renewal once active breakouts have resolved.

7. Can I use Professional Serum during pregnancy or while breastfeeding?

While Professional Serum has not been specifically studied in pregnant or breastfeeding women, it contains naturally derived bioactive proteins and is free from added fragrance and harsh chemical actives commonly found in many skincare products.

Because of its gentle formulation and long history of professional use, many physicians and aesthetic practitioners choose to incorporate Professional Serum into skincare routines during pregnancy and breastfeeding.

As with any skincare product, we recommend discussing its use with your physician or healthcare provider, who can advise you based on your individual circumstances.

8. Are there any products or ingredients I should avoid while using Professional Serum?

Professional Serum is compatible with most skincare routines and can generally be used alongside other skincare products.

For best results, apply it to clean skin immediately after cleansing and before other leave-on products, allowing the bioactive ingredients to absorb directly into the skin.

If you are recovering from a professional aesthetic procedure, always follow your treating physician’s or aesthetic practitioner’s post-procedure instructions regarding which skincare products should be used during your recovery.

From smoother, healthier-looking skin to enhanced recovery after aesthetic procedures, here’s what you can realistically expect—and when you can expect to see it.

1. What skin concerns can Professional Serum help improve?

Professional Serum is formulated to address multiple visible signs of skin aging, including:

• Fine lines and wrinkles


• Loss of firmness and elasticity


• Uneven skin tone and texture


• Dull-looking skin


• Visible signs of photoaging

Professional Serum was also specifically developed for use following aesthetic procedures. Treatments such as microneedling, laser resurfacing, and chemical peels work by creating controlled micro-injuries that activate the skin’s natural repair response.

During this period of heightened repair activity, Professional Serum delivers a high concentration of PTT-6^® bioactive signaling molecules to help support the skin’s natural recovery process. This helps optimize the skin’s response to treatment, promotes healthier-looking recovery, and may help reduce visible downtime following many professional procedures.

2. When will I start seeing visible results?

Many people notice that their skin feels smoother, more hydrated, and more radiant within the first few weeks of consistent use.

Visible improvements in skin firmness, elasticity, texture, and the appearance of fine lines typically become more noticeable with continued daily use as the skin’s natural renewal process continues over time.

If Professional Serum is used following an aesthetic procedure, many patients also notice that their skin appears calmer and recovers more comfortably during the days after treatment.

3. What improvements should I expect first?

The first changes people commonly notice are improvements in skin hydration, softness, and overall radiance.

With continued use, skin often appears smoother, firmer, and more resilient, while fine lines, uneven texture, and other visible signs of aging gradually become less noticeable.

Following professional treatments, many users also report healthier-looking skin recovery and less visible downtime as the skin progresses through its natural healing process.

4. Can Professional Serum help improve fine lines, wrinkles, skin firmness, and elasticity?

Yes.

Professional Serum delivers the highest concentration of PTT-6^® in the CALECIM^® skincare range to support the skin’s natural renewal process.

With consistent use, it helps improve the appearance of fine lines and wrinkles while promoting firmer, smoother, and more resilient-looking skin. As skin renews itself over time, improvements in elasticity and overall skin quality become increasingly noticeable.

5. Can Professional Serum improve uneven skin tone, rough texture, and dull-looking skin?

Yes.

Healthy skin renewal is essential for maintaining smooth texture and a more even-looking complexion.

By supporting the skin’s natural regenerative processes, Professional Serum helps refine rough texture, improve radiance, and promote healthier-looking skin with continued use. Many users notice that their skin appears brighter, smoother, and more refreshed over time.

6. Can Professional Serum support skin recovery after aesthetic procedures?

Yes.

Professional Serum was originally developed for physicians and aesthetic practitioners to use following procedures that intentionally create controlled micro-injuries, including microneedling, laser resurfacing, RF microneedling, and chemical peels.

These treatments work by activating the skin’s natural repair mechanisms. Professional Serum complements this process by delivering a high concentration of PTT-6^® bioactive signaling molecules precisely when the skin is in an active state of renewal.

By supporting the skin’s natural recovery process during this critical window, Professional Serum helps optimize the benefits of the procedure, promotes healthier-looking skin recovery, and may help reduce visible downtime while the skin repairs itself.

Many practitioners also recommend continuing Professional Serum at home for several days after treatment to extend post-procedure care while the skin continues to regenerate.

7. Will my results continue to improve with ongoing use?

Yes.

Skin renewal is a continuous biological process. Every day, your skin responds to environmental stressors such as UV exposure, pollution, and oxidative stress while naturally producing less collagen and elastin as it ages.

Using Professional Serum consistently helps support your skin’s daily renewal and repair processes. As a result, many people continue to see improvements in overall skin quality, firmness, texture, and radiance with ongoing use.

Like regular exercise for your skin, consistency delivers the greatest long-term benefits.

8. What happens if I stop using Professional Serum?

Professional Serum supports your skin’s natural renewal processes—it does not permanently change how your skin ages.

If you stop using the serum, your skin will gradually return to its normal rate of aging as it continues to be affected by time and everyday environmental stressors.

Continuing regular use helps maintain the visible improvements you’ve achieved and provides ongoing support for healthier-looking, more resilient skin over the long term.